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( a–c ) Representative triple stain images for N1ICD, <t>Notch1,</t> and Jag2 mRNA in sebaceous glands (SGs) from mice (n=5 each) treated with aRW, 3 d post-treatment. ( a ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( b ) Two channels showing N1 ISH and J2 ISH. ( c ) One channel showing N1ICD. ( d-f ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with aJ1, 3 d post-treatment. ( d ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( e ) Two channels showing N1 ISH and J2 ISH. ( f ) One channel showing N1ICD. ( g ) Quantification of the percentage of N1ICD positive (N1ICD+) basal stem cells in SGs from mice (n=5 each) treated with aRW, aJ1, and aJ2, 3 d post-treatment. Percentage was calculated by dividing the number of N1ICD+ basal stem cells by the total number of basal stem cells in each SG. ( h–j ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with aJ2, 3 d post-treatment. ( h ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( i ) Two channels showing N1 ISH and J2 ISH. ( j ) One channel showing N1ICD. ( k ) Quantification of what percentage of the N1ICD+ basal stem cells express both N1 ISH and J2 ISH, only N1 ISH, or only J2 ISH. ( g and k ) Total n of SGs quantified per treatment: aRW=13, aJ1=15, aJ2=15. Error bars represent SEM. Scale bars are 25 μm. Figure 2—source data 1. Source data for .
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( a–c ) Representative triple stain images for N1ICD, <t>Notch1,</t> and Jag2 mRNA in sebaceous glands (SGs) from mice (n=5 each) treated with aRW, 3 d post-treatment. ( a ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( b ) Two channels showing N1 ISH and J2 ISH. ( c ) One channel showing N1ICD. ( d-f ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with aJ1, 3 d post-treatment. ( d ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( e ) Two channels showing N1 ISH and J2 ISH. ( f ) One channel showing N1ICD. ( g ) Quantification of the percentage of N1ICD positive (N1ICD+) basal stem cells in SGs from mice (n=5 each) treated with aRW, aJ1, and aJ2, 3 d post-treatment. Percentage was calculated by dividing the number of N1ICD+ basal stem cells by the total number of basal stem cells in each SG. ( h–j ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with aJ2, 3 d post-treatment. ( h ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( i ) Two channels showing N1 ISH and J2 ISH. ( j ) One channel showing N1ICD. ( k ) Quantification of what percentage of the N1ICD+ basal stem cells express both N1 ISH and J2 ISH, only N1 ISH, or only J2 ISH. ( g and k ) Total n of SGs quantified per treatment: aRW=13, aJ1=15, aJ2=15. Error bars represent SEM. Scale bars are 25 μm. Figure 2—source data 1. Source data for .
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( a–c ) Representative triple stain images for N1ICD, <t>Notch1,</t> and Jag2 mRNA in sebaceous glands (SGs) from mice (n=5 each) treated with aRW, 3 d post-treatment. ( a ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( b ) Two channels showing N1 ISH and J2 ISH. ( c ) One channel showing N1ICD. ( d-f ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with aJ1, 3 d post-treatment. ( d ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( e ) Two channels showing N1 ISH and J2 ISH. ( f ) One channel showing N1ICD. ( g ) Quantification of the percentage of N1ICD positive (N1ICD+) basal stem cells in SGs from mice (n=5 each) treated with aRW, aJ1, and aJ2, 3 d post-treatment. Percentage was calculated by dividing the number of N1ICD+ basal stem cells by the total number of basal stem cells in each SG. ( h–j ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with aJ2, 3 d post-treatment. ( h ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( i ) Two channels showing N1 ISH and J2 ISH. ( j ) One channel showing N1ICD. ( k ) Quantification of what percentage of the N1ICD+ basal stem cells express both N1 ISH and J2 ISH, only N1 ISH, or only J2 ISH. ( g and k ) Total n of SGs quantified per treatment: aRW=13, aJ1=15, aJ2=15. Error bars represent SEM. Scale bars are 25 μm. Figure 2—source data 1. Source data for .
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Image Search Results


Journal: eLife

Article Title: Nicotine enhances the stemness and tumorigenicity in intestinal stem cells via Hippo-YAP/TAZ and Notch signal pathway

doi: 10.7554/eLife.95267

Figure Lengend Snippet:

Article Snippet: Antibody , mouse monoclonal anti-Notch1 , Santa Cruz , #sc-376403 , WB (1:100).

Techniques:

( a–c ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in sebaceous glands (SGs) from mice (n=5 each) treated with aRW, 3 d post-treatment. ( a ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( b ) Two channels showing N1 ISH and J2 ISH. ( c ) One channel showing N1ICD. ( d-f ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with aJ1, 3 d post-treatment. ( d ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( e ) Two channels showing N1 ISH and J2 ISH. ( f ) One channel showing N1ICD. ( g ) Quantification of the percentage of N1ICD positive (N1ICD+) basal stem cells in SGs from mice (n=5 each) treated with aRW, aJ1, and aJ2, 3 d post-treatment. Percentage was calculated by dividing the number of N1ICD+ basal stem cells by the total number of basal stem cells in each SG. ( h–j ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with aJ2, 3 d post-treatment. ( h ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( i ) Two channels showing N1 ISH and J2 ISH. ( j ) One channel showing N1ICD. ( k ) Quantification of what percentage of the N1ICD+ basal stem cells express both N1 ISH and J2 ISH, only N1 ISH, or only J2 ISH. ( g and k ) Total n of SGs quantified per treatment: aRW=13, aJ1=15, aJ2=15. Error bars represent SEM. Scale bars are 25 μm. Figure 2—source data 1. Source data for .

Journal: eLife

Article Title: The Jag2/Notch1 signaling axis promotes sebaceous gland differentiation and controls progenitor proliferation

doi: 10.7554/eLife.98747

Figure Lengend Snippet: ( a–c ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in sebaceous glands (SGs) from mice (n=5 each) treated with aRW, 3 d post-treatment. ( a ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( b ) Two channels showing N1 ISH and J2 ISH. ( c ) One channel showing N1ICD. ( d-f ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with aJ1, 3 d post-treatment. ( d ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( e ) Two channels showing N1 ISH and J2 ISH. ( f ) One channel showing N1ICD. ( g ) Quantification of the percentage of N1ICD positive (N1ICD+) basal stem cells in SGs from mice (n=5 each) treated with aRW, aJ1, and aJ2, 3 d post-treatment. Percentage was calculated by dividing the number of N1ICD+ basal stem cells by the total number of basal stem cells in each SG. ( h–j ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with aJ2, 3 d post-treatment. ( h ) Four channels showing N1ICD, N1 ISH, J2 ISH, and DAPI. ( i ) Two channels showing N1 ISH and J2 ISH. ( j ) One channel showing N1ICD. ( k ) Quantification of what percentage of the N1ICD+ basal stem cells express both N1 ISH and J2 ISH, only N1 ISH, or only J2 ISH. ( g and k ) Total n of SGs quantified per treatment: aRW=13, aJ1=15, aJ2=15. Error bars represent SEM. Scale bars are 25 μm. Figure 2—source data 1. Source data for .

Article Snippet: Antibody , Anti-Notch1 (Human monoclonal) , Genentech , , Inhibiting antibody (5 mg/kg).

Techniques: Staining

(Related to ). ( a–c ) Quantification of the location of N1ICD+ basal stem cells in sebaceous glands (SGs) from mice (n=5 each) treated with aRW ( a ), aJ1 ( b ), and aJ2 ( c ), 3 d post-treatment. The SG was divided into five sections along the proximal-distal axis. The number of N1ICD+ basal stem cells in each section was divided by the total number of N1ICD+ basal stem cells in each SG. Total n of SGs quantified per treatment: aRW=13, aJ1=15, aJ2=15. ( d ) Quantification of what percentage of the total basal stem cells (N1ICD+ and N1ICD-) express both N1 ISH and J2 ISH. Total n of SGs quantified per treatment: aRW=13, aJ1=15, aJ2=15. ( e,f ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with ( e ) aRW and ( f ) aJ2, 3 d post-treatment. ( g ) Quantification of the width of the interfollicular epidermis (IFE), 7 d after treatment with aRW, aJ1, aJ2, and aJ1J2. p-values: aJ1=0.528, aJ2=0.885, aJ1J2=0.840. Total n of SGs quantified per treatment: aRW=26, aJ1=27, aJ2=27, aJ1J2=22. ( h ) Quantification of the width of the adipocyte layer, 7 d after treatment with aRW, aJ1, aJ2, and aJ1J2. p-values: aJ1=0.890, aJ2=0.948, aJ1J2=0.374. Total n of SGs quantified per treatment: aRW=13, aJ1=13, aJ2=15, aJ1J2=15. ( i,j ) Representative hematoxylin and eosin (H&E) images of SGs from mice (n=5 each) treated with aRW ( i ) and aJ2 ( j ), 7 d post-treatment. Student’s t-test used for statistical analysis. Error bars represent SEM. Scale bars are 100 μm. Figure 2—figure supplement 1—source data 1. Source data for .

Journal: eLife

Article Title: The Jag2/Notch1 signaling axis promotes sebaceous gland differentiation and controls progenitor proliferation

doi: 10.7554/eLife.98747

Figure Lengend Snippet: (Related to ). ( a–c ) Quantification of the location of N1ICD+ basal stem cells in sebaceous glands (SGs) from mice (n=5 each) treated with aRW ( a ), aJ1 ( b ), and aJ2 ( c ), 3 d post-treatment. The SG was divided into five sections along the proximal-distal axis. The number of N1ICD+ basal stem cells in each section was divided by the total number of N1ICD+ basal stem cells in each SG. Total n of SGs quantified per treatment: aRW=13, aJ1=15, aJ2=15. ( d ) Quantification of what percentage of the total basal stem cells (N1ICD+ and N1ICD-) express both N1 ISH and J2 ISH. Total n of SGs quantified per treatment: aRW=13, aJ1=15, aJ2=15. ( e,f ) Representative triple stain images for N1ICD, Notch1, and Jag2 mRNA in SGs from mice (n=5 each) treated with ( e ) aRW and ( f ) aJ2, 3 d post-treatment. ( g ) Quantification of the width of the interfollicular epidermis (IFE), 7 d after treatment with aRW, aJ1, aJ2, and aJ1J2. p-values: aJ1=0.528, aJ2=0.885, aJ1J2=0.840. Total n of SGs quantified per treatment: aRW=26, aJ1=27, aJ2=27, aJ1J2=22. ( h ) Quantification of the width of the adipocyte layer, 7 d after treatment with aRW, aJ1, aJ2, and aJ1J2. p-values: aJ1=0.890, aJ2=0.948, aJ1J2=0.374. Total n of SGs quantified per treatment: aRW=13, aJ1=13, aJ2=15, aJ1J2=15. ( i,j ) Representative hematoxylin and eosin (H&E) images of SGs from mice (n=5 each) treated with aRW ( i ) and aJ2 ( j ), 7 d post-treatment. Student’s t-test used for statistical analysis. Error bars represent SEM. Scale bars are 100 μm. Figure 2—figure supplement 1—source data 1. Source data for .

Article Snippet: Antibody , Anti-Notch1 (Human monoclonal) , Genentech , , Inhibiting antibody (5 mg/kg).

Techniques: Staining

Journal: eLife

Article Title: The Jag2/Notch1 signaling axis promotes sebaceous gland differentiation and controls progenitor proliferation

doi: 10.7554/eLife.98747

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-Notch1 (Human monoclonal) , Genentech , , Inhibiting antibody (5 mg/kg).

Techniques: Control, Staining, Sequencing, RNAscope, Multiplex Assay